논문검색
Author Jae Ho Byun, M.D.1, Young Seon Hong, M.D.1, Hee-Jeong Cheong, M.S.2, Sook-Ja Kim,2 Nam-Su Lee, M.D.2, Jong-Ho Won, M.D.2, Dae-Sik Hong, M.D.2 and Hee-Sook Park, M.D.2
Place of duty 1St. Mary's Hospital, Division of Oncology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea; 2Division of Hematology-oncology, Department of Internal Medicine and Institute for Clinical Molecular Biology Research, SoonChunHyang University College of Medicine, Seoul, Korea
Title Effect of Arsenic Trioxide in TRAIL (Tumor Necrosis Factor-related Apoptosis Inducing Ligand)-Mediated Apoptosis in Multiple Myeloma Cell Lines
Publicationinfo Cancer Research and Treatment 2003 Dec; 035(06): 472-477.
Key_word Arsenic trioxide, Multiple myeloma, Apoptosis, Caspase
Full-Text
Abstract Purpose: The potential therapeutic application of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), in the treatment of multiple myeloma (MM), was recently proposed. However, there have been some problems with the use of TRAIL, due to the appearance of TRAIL-resistant cells in MM. The effect of arsenic trioxide (As2O3) on the rate of apoptosis induced by TRAIL was evaluated in MM cells.

Materials and Methods: Using TRAIL-sensitive (RPMI- 8226) and TRAIL-resistant (ARH-77 and IM-9) MM cell lines, the cell viability, induction of apoptosis, and change in the caspases were examined after treatment with TRAIL alone, or in combination with various concentrations of As2O3.

Results: Incubating the cell lines with As2O3 augmented the TRAIL-induced apoptosis in the MM cell lines, according to the As2O3 concentration. Apoptosis was mediated through caspase activation. When TRAIL was used alone, caspase8 was activated in the RPMI-8226 cell lines, but not in the ARH-77 and IM-9 cell lines. When As2O3 was added to TRAIL, caspase-9 was activated in the ARH-77 and IM-9 cells.

Conclusion: The use of As2O3, in combination with TRAIL, would help enhance the level of TRAIL-induced apoptosis, and overcome the TRAIL-resistance, in MM cells. (Cancer Research and Treatment 2003;35:472⁣477)